Many still advocate for hps and mh grow lights, but in recent years, led grow lights have become. The following document is intended to demonstrate setup of this instrument. The spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Spectramax m5 plate reader proteomics and mass spectrometry. In addition, the id5 reader measures trf and fp and can be expanded to include trfret, htrf, bret, dual luciferase reporter assays with injectors, and western blot detection. Spectramax 190 microplate spectrophotometer operators manual iii labor and parts to obtain warranty service during the applicable warranty period, you must take the product or deliver the product properly packaged in the original shipping materials and carton to an authorized molecular devices corporation service facility. Spectramax plus 384 microplate reader manual conquer scientific. The spectramax i3x microplate reader from molecular devices evolves with your future research needs and offers an unlimited breadth of applications the spectramax i3x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality.
Download spectramax m3, m4, m5, and m5 e multimode microplate readers book pdf free download link or read online here in pdf. Molecular devices spectramax 190 microplate reader includes softmax. Spectramax m3, m4, m5, and m5 e multimode microplate. Optics mirrored optics focus the light into the sa mple volume, and cutoff filters are used to. Located at the right front of the spectramax plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. All spectramax m series multimode microplate readers measure uv and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard.
Read online spectramax m3, m4, m5, and m5e multimode microplate. Optics mirrored optics focus the light into the sample volume, and cutoff filters are used to. For very concentrated samples, use a minimum volume. The broad array of spectramax i3x detection cartridges enables the user. The spectramax m2 and spectramax m2e multi detection readers are monochromator based microplate readers that have 6 well to 384 well. The spectramax i3x multimode microplate reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. In this user guide, all references to spectramax multimode. Softmax pro software user guide biological discovery in. Softmax pro software has been integrated by many leading robotics and. The spectramax id3 and id5 multimode microplate readers measure absorbance, fluorescence, and luminescence.
Microplate readers, multimode, spectramax m series vwr. In no event will mdc be liable to the user for any damages, including any lost profits, lost savings, or other. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Spectramax i3 multimode platform a flexible, userupgradeable microplate detection system key features the spectramax i3 platform from molecular devices is a multimode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Softmax pro data acquisition and analysis software user guide. The spectramax m2 and m2e readers provide two detection modes in one platform, and offer the the added functionality of bottomread fluorescence for cellbased assays m2e. This revolution in modularity makes nocompromise detection the norm.
The m2 can scan across a range of wavelengths in 1 nm increments and a single read command can run a combined absorbance and fluorescence intensity assay. Manuals, cables, power cords, software, drivers, accessories, etc are not included unless pictured or noted in the description. Spectramax i3 spectratest lm1 protocol softmax pro. Spectramax led lights minimize cost, maximize light. If the plate is new, or has been recertified since the last calibration, the new absorbance values must. Plates are used to validate optical performance of spectramax m3, m4, and. Spectramax plus 384 microplate reader manual conquer. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors.
Patient care, professional education and research are carried out at hospitals and. Rapid, accurate dna, rna, and protein quantitation in a one touch, full spectrum micro volume absorbance reader. The spectramax m3 multimode microplate reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch. Molecular devices spectramax m2 multimode microplate reader.
Read online spectramax m3, m4, m5, and m5 e multimode microplate readers book pdf free download link book now. The spectramax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Spectramax m3, m4, m5, and m5 e multimode microplate readers user guide 01120115 f july 2010. The molecular devices spectramax m3 multimode microplate reader covers a wide range of applications without the need to change filters. Spectramax i3 spectratest fl1 protocol softmax pro. The molecular devices spectramax m5m5e microplate reader was tested for compatibility with life technologies geneblazer assays. Detection modalities include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to a topoftheline dedicated spectrofluorometer or spectrophotometer. With optimized reagents and the industryleading data acquisition and analysis tool softmax pro 7 software, the spectramax id3 allows customization of workflow to. This dual monochromatic, multi mode microplate reader delivers three modes of detection, uv vis absorbance reading, fluorescence and luminescence, and a broad spectrum of formats from cuvettes to 6, 12, 24, 48, 96, and 384well microplates.
The spectramax 190 reader is the tool for dna analysis. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. For fluorescence intensity assays, users can select from top or bottomread modes for improved sensitivity in solution and cellbased assays. Spectramax microplate spectrophotometer operators manual. The spectramax m3 microplate reader is the standard for uvvisible multimode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6, 12, 24, 48, 96, and 384well microplates. Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence.
Softmax pro software user guide marine biological laboratory. This easy to use multimode plate reader is designed to handle the work flow of multiple research projects. Spectramax id3 and id5 multimode microplate readers. With the spectramax minimax 300 imaging cytometer, industryleading softmax pro software, and user. Future proof your discoveries and let the spectramax i3x reader evolve with your research. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The ut health science center campuses include colleges of dentistry, graduate health sciences, health professions, medicine, nursing and pharmacy.
The upgradeable spectramax m5 multimode microplate reader comes with the standard five modes of detection with the upgrade option to add htrf at a later time. Using the molecular devices test plate to calibrate the. Spectramax m3 reader spectramax m4 reader spectramax m5 reader spectramax m5e reader detection modes. Spectramax m3 multimode microplate reader, molecular devices. Catchpoint simplestep elisa kits give users a 90minute elisa workflow, a nearly twothirds reduction in time compared to conventional elisas.
Molecular devices spectramax i3 manual nova scotia. Detection modes include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to. The spectramax m series multimode microplate readers measure uv and visible absorbance, fluorescence, luminescence, fluorescence polarization, trf and htrf. Molecular devices spectramax plus384 microplate reader manual attachments molecular devices spectramax plus384 microplate reader manual 624 kb. Molecular devices spectramax m3 multimode microplate reader 90 day warranty. Luciferase detection instrumentation guide thermo fisher.
Spectramax plus 384 microplate spectrophotometer operators manual vii conventions used in this manual the names of keys that appear on the spectramax plus 384 control panel are shown in boxed helvetica type. Software for the test plate must be installed on the control computer before the plate can be used. Spectramax m3 multimode microplate reader, see page 202. Molecular devices just launched spectramax id3 multimode microplate reader is the cornerstone of complete laboratory solution helping to expand the boundaries of research. Spectramax id3 multimode microplate reader the spectramax id3 multimode microplate reader is the cornerstone of a. Apr 07, 2015 molecular devices spectramax plus 384 uv absorbance microplate reader harlow scientific. Spectramax i3 spectratest lm1 protocol evelyn mcgown 20726 16.
The ability to detect 16 ngwell of dna and to quantitate 50 ng of dna provides the same sensitivity as a spectrophotometer. Spectramax m3 multimode microplate reader, see page 122. Spectramax m5 microplate reader from molecular devices llc. For more detailed information and technical support of molecular devices instruments or software. Compatible automation solutions for spectramax readers. Spectramax m series multimode microplate readers 7 the spectradrop microvolume microplate offers the ability to use as little as 2l samples with 24 or 64well plates. Many still advocate for hps and mh grow lights, but in recent years, led grow lights have become much more advanced than when they first came into the market. Userupgradeable applications userexchangeable detection modules expand the systems detection capabilities to include timeresolved fluorescence, htrf, fast kinetics with injectors, and western blot detection.
These settings are also valid for the spectramax m3m4, flexstation 3 multimode. Robust, highvalue microplate readers can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays. Spectramax m3 microplate reader from molecular devices llc description the spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The spectramax i3x multimode detection platform utilizes a patentpending design that allows for realtime system configuration by the user in less than two minutes. These robust readers have been placed in labs from antarctica to the. Spectramax m2m2e user guide university of michigan. Molecular devices spectramax m5 multimode microplate. Molecular devices spectramax id3 multimode microplate. Spectramax 190 microplate spectrophotometer operators manual vii conventions used in this manual the names of keys that appear on the spectramax 190 control panel are shown in boxed helvetica type. The cuvette chamber spectramax plus 384 only figure 1.
Spectramax i3 spectratest fl1 protocol evelyn mcgown 20726 16. Spectratest fl1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information. Spectramax m3, m4, m5, and m5e multimode microplate reader. Spectramax m series microplate readers, cuvette port, iq oq. Setup guide on the molecular devices spectramax m5m5e. Spectramax i3 multimode detection platform youtube. With optimized reagents, option to operate the readers. The spectramax m3 multimode microplate reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Multimode microplate reader spectra max m3 climate impacts. Microplate readers include the m3, m4, m5, and m5e models. We therefore gathered salient information about many of the instruments available to date. Download spectramax m3, m4, m5, and m5e multimode microplate. All books are in clear copy here, and all files are secure so dont worry about it.
The molecular devices spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. User manual spectramax m3 m4 m5 m5e software manual softmax pro this entry was posted in equipment, facility news, resources and tagged fluorescence plate reader, plate reader, protein concentration, shared equipment, spectramax m5 plate reader on may 16, 2014 by tania. The pathcheck sensor can extend the instrument linearity by a factor of three or more. The spectramax m2 is a highly flexible dual monochromator, multimode microplate reader with full spectral range detection for cuvettes and 6 to 384 well microplates.
Dec 03, 20 the spectramax minimax 300 imaging cytometer enables cell visualization and cellbased analysis on the spectramax i3 multimode microplate reader. Spectramax m3, m4, m5, and m5 e multimode microplate readers. Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence for each well of a microplate or from the cuvette port. Spectramax m3 microplate reader from molecular devices llc. Spectramax m3, m4, m5, and m5e multimode microplate. Molecular devices spectramax id3 multimode microplate reader. Spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability.
Spectratest lm1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information. Introduction softmax pro software controls molecular devices spectrophotometers, absorbance, luminescence, and fluorescence microplate re adersproviding extensive data calculation and analysis capabilities under a glpgmp work environment for pharmaceutical, bio. Specifications see manual for details does uv and visible absorbance, and fluorescence intensity measurements on microplates and. The spectramax m5 multimode microplate reader delivers single mode reader performance in one multimode reader package. Established in 1911, the university of tennessee health science center aims to improve human health through education, research, clinical care and public service. Operators manual molecular devices spectramax 190 absorbance plate reader. Unit powers up but is not tested for any analytical application, we dont run any liquid. Spectramax m series multimode microplate readers molecular. Introduction the spectramax m2 and spectramax m2 e multidetection readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well. Molecular devices spectramax m3 multi mode microplate reader. Spectramax id3 multimode microplate reader molecular. Spectramax and versamax plate readers operators manual 01120126 rev a 5 1. To view the softmax pro user guide, the formula reference guide, or the.
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